A polymer-dispersed liquid crystal composition, a preparation method of a polymer-dispersed liquid crystal film, and a display panel are provided. The polymer-dispersed liquid crystal composition includes a prepolymer and a liquid crystal molecule, wherein the prepolymer is a fluorine-containing polyimide precursor chemically bonded to a polyaniline, including a polycondensation unit formed from a fluorine-containing dianhydride monomer and a fluorine-containing diamine monomer. In the present application, the conductive polyaniline is chemically bonded to the fluorine-containing polyimide precursor to increase the conductivity of the polyimide precursor. Further, a polymer-dispersed liquid crystal film having a driving voltage lower than 30 V and excellent insulating property and radiation resistance is obtained.