Invention Grant
- Patent Title: Methods for removal of adaptor dimers from nucleic acid sequencing preparations
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Application No.: US16263726Application Date: 2019-01-31
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Publication No.: US11519026B2Publication Date: 2022-12-06
- Inventor: Arkadiusz Bibillo , Randall W. Davis
- Applicant: Roche Sequencing Solutions, Inc.
- Applicant Address: US CA Pleasanton
- Assignee: Roche Sequencing Solutions, Inc.
- Current Assignee: Roche Sequencing Solutions, Inc.
- Current Assignee Address: US CA Pleasanton
- Agent Eric Grant Lee; Olga Kay
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6869 ; C12Q1/6855 ; C12Q1/6806 ; C12Q1/6848
Abstract:
Sequencing adaptors and methods are provided for preparation of polynucleotides for sequencing. The sequencing adaptors contain a portion of a recognition sequence for a methyl-dependent endonuclease. Unwanted adaptor dimers that form during ligation of adaptors to target polynucleotides produce a complete restriction sequence and are cleaved by the endonuclease, followed by exonuclease digestion, thereby removing the dimers.
Public/Granted literature
- US20190161794A1 METHODS FOR REMOVAL OF ADAPTOR DIMERS FROM NUCLEIC ACID SEQUENCING PREPARATIONS Public/Granted day:2019-05-30
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