A golf course route guiding system includes a controller disposed on a vehicle and having a memory module, a positioning module, and a processing module. The memory module stores a field information of a golf course and a plurality of movement zones set corresponding to the field information. Each movement zone has a route range. The positioning module provides a location signal indicating a current location of the vehicle. The processing module receives the location signal and determines the movement zone in which the vehicle is located, and assigns a route range set in the corresponding movement zone, which is sent to the processing module, so that the processing module limits the vehicle to move in the assigned route range. When the vehicle enters another movement zone, the processing module reassigns a route range, achieving a dynamic route adjustment function.