A display substrate, a display apparatus, and a manufacturing method of the display substrate are provided. The display substrate includes: a base substrate; and a crystallization induction layer and a polysilicon layer stacked on the base substrate. The crystallization induction layer includes induction layer patterns and intervals between the induction layer patterns. The polysilicon layer includes a portion overlapping the induction layer patterns and a portion overlapping the intervals, a crystallinity of the portion of the polysilicon layer overlapping the induction layer patterns is larger than a crystallinity of the portion of the polysilicon layer overlapping the intervals.