The present disclosure provides an information tamper-resistant system and method. The system includes: a storage module; a writing module connected with the storage module through a first OTP switch, to write source information to the storage module; a first reading module connected with the storage module through a second OTP switch, to read out written information in the storage module and disconnect the first OTP switch and the second OTP switch after confirming that the written information is accurate; and a second reading module connected with the storage module through a third OTP switch, to read out information stored in the storage module after the third OTP switch is switched on; the first OTP switch, the second OTP switch, and the third OTP switch can only perform one switch-on operation or one switch-off operation. The system and method effectively avoid theft and tampering of information.