A reverse polarity protection device includes a protection unit, a detection unit, and a control unit electrically connected between a power supply device and a load device. The detection unit is electrically connected to the power supply device for detecting the polarity of an output signal of the power supply device, and the control unit is electrically connected to the detection unit and the protection unit. The detection unit outputs a detection signal to the control unit according to a detection result of the polarity of the output signal. If the detection signal shows that the polarity of the output signal is reverse, the control unit will control the protection unit to form an open circuit between the power supply device and load device to stop transmitting the output signal of the power supply device to the load device and achieve a reverse polarity protection effect of the load device.