A display substrate including a base substrate having a display area and a non-display area around the display area, a plurality of gate lines disposed in the display area and extending in a first direction, a plurality of data lines disposed in the display area, insulated from the gate lines, and extending in a second direction crossing the first direction, and a plurality of data line extension portions extending from the data lines to the non-display area, in which each of the data line extension portions includes a first portion and a second portion disposed at a higher position than the first portion.