Disclosed is a blood flow measurement apparatus using Doppler ultrasound. The apparatus includes a two-dimensional transducer array in which a plurality of transducers are two-dimensionally arranged, an acoustic window detection portion configured to transmit and receive ultrasonic signals by driving some of the plurality of transducers, to detect Doppler signals, and to confirm a transducer corresponding to a Doppler signal having high intensity among the detected Doppler signals, a blood flow detection portion configured to detect Doppler signals with respect to a plurality of steering vectors through beam steering using a plurality of adjacent transducers including the confirmed transducer and configured to confirm a steering vector corresponding to a Doppler signal having highest intensity among the detected Doppler signals, and a Doppler processing portion configured to detect a Doppler signal by performing beam steering using the confirmed steering vector and to obtain blood flow information from the detected Doppler signal.