The subject matter described herein includes methods, systems, and computer readable media for proactive network testing. One method for proactive network testing includes receiving, by a test controller and via a network tap, at least one metric associated with live network traffic; determining, by the test controller and using the at least one metric and a threshold value associated with the at least one metric, that a network test is to be performed; configuring, by the test controller, a first test agent to execute the network test; and executing, by the first test agent, the network test.