Described is a mixer for a chromatography system. The mixer includes an inlet manifold channel, an outlet manifold channel and a plurality of transfer channels. The inlet manifold channel has an inlet at a proximal end of the inlet manifold channel for receiving an inlet flow. The transfer channels are fluidly connected between the inlet and outlet manifold channels. The respective fluid connections are distributed along each of the inlet and outlet manifolds channels. The transfer channels have different volumes. The mixer may be formed of a plurality of layer and the layers may be diffusion bonded to each other.