Methods, systems, and media for timing presentation of content are provided. In some embodiments, the method comprises: causing a first video content item to be presented on a user device of a user, wherein the video content item is provided by a video sharing service; identifying a promotion for the video sharing service that is to be presented to the user during a time window; determining a probability that the user will select the promotion if the promotion is presented at a first time point within the time window in connection with the first video content item; determining whether to present the promotion at the first time point based on at least on the probability; in response to determining that the promotion is to be presented at the first time point, causing the promotion to be presented on the user device at the first time point in connection with the first video content item; and, in response to determining that the promotion is not to be presented at the first time point, causing the promotion to be presented on the user device at a second time point in connection with a second video content item, wherein the second time point is within the time window and wherein the second time point is after the first time point.