Provided are a method of adjusting uplink timing in a wireless communication system and an apparatus therefor. Specifically, a method for a user equipment to adjust uplink timing in a wireless communication system includes transmitting, to a base station, an uplink signal, receiving, from the base station, a timing advance (TA) command configured based on the uplink signal, and performing uplink transmission to the base station by applying a timing advance (TA) indicated by the TA command. The TA command may be interpreted based on subcarrier spacing of at least one frequency resource region to which the TA is to be applied.