A dishwasher appliance includes a cabinet having a wash chamber for receipt of articles for cleaning, a rack assembly slidably received into the wash chamber and configured for the receipt of the articles, and a fluid dispensing assembly for dispensing a fluid onto the articles in the rack assembly during operation of the dishwasher appliance. Further, the dishwasher appliance includes a spot detection assembly. The spot detection assembly includes a transparent component and an imaging device arranged adjacent to the transparent component. As such, the imaging device is configured to detect one or more fluid spots on the transparent component, which is indicative of fluid spots being present on the articles.