A manufacturing method of a semiconductor device at least includes the following steps. A substrate having a stacked structure is provided. An epitaxy process is performed to form an epitaxial layer on the substrate on two sides of the stacked structure. A recess is forming on the two sides of the stacked structure, wherein the recess penetrates through the epitaxial layer, extends into the substrate, and has a tip located in the substrate. A source/drain region is formed in the recess, wherein a material of the source/drain region comprises silicon germanium. A spacer wall material layer is formed on the substrate. A portion of the stacked structure is removed to from a gate structure. A portion of the spacer wall material layer is removed to form a spacer wall on the epitaxial layer. A semiconductor device is also provided.