A display substrate, a manufacturing method therefor and a pixel driving circuit, the display substrate includes: a base substrate; a first conductive layer, which includes a first signal line, a second signal line, and an additional pad layer, on the base substrate; a pixel defining layer on the first conductive layer and having an opening; and an electroluminescent material layer in the opening and including a first end portion and a second end portion, an orthographic projection of the first end portion on the base substrate falls within that of the first signal line, an orthographic projection of the second end portion on the base substrate falls within that of the additional pad layer, and the orthographic projections of the first end portion and the second end portion are respectively located on both sides of an orthographic projection of the second signal line on the base substrate.