The present invention reveals a needle set of laparoscopic surgery and knotting device thereof, the needle set comprising a body and an axis sleeve. Near the body side is formed a barrier, and the other side have a concave groove. The axis sleeve is hollow tube and fixedly run through the body. Therefore the needle set can axial insert into to the axis sleeve, and achieve the effect that minimizing the complicate of overall structure, reducing the length of a knotting device, and more convenient to operate.