Disclosed is a checksum generation and validation system and associated methods for dynamically generating and validating checksums with customizable levels of integrity verification. The system receives a file with data points defined with positional values and non-positional values, and differentiates a first set of the data points from a second set of the data points. The system generates a checksum based on a combination of two or more values from the positional values and the non-positional values of each data point from the first set of data points, and further based on exclusion of the positional values and the non-positional values of the second set of data points from the checksum. The system may use the checksum to verify the integrity of the data associated with the first set of data points.