Systems and methods for super-resolution ultrasound imaging of microvessels in a subject are described. Ultrasound data are acquired from a region-of-interest in a subject who has been administered a microbubble contrast agent. The ultrasound data are acquired while the microbubbles are moving through, or otherwise present in, the region-of-interest. The region-of-interest may include, for instance, microvessels or other microvascuiature in the subject. By isolating, localizing, tracking, and accumulating the microbubbles in the ultrasound data, super-resolution images of the microvessels can be generated.