Invention Grant
- Patent Title: DNA methylation editing kit and DNA methylation editing method
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Application No.: US16801969Application Date: 2020-02-26
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Publication No.: US11591623B2Publication Date: 2023-02-28
- Inventor: Izuho Hatada , Sumiyo Morita , Takuro Horii
- Applicant: NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY
- Applicant Address: JP Gunma
- Assignee: NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY
- Current Assignee: NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY
- Current Assignee Address: JP Gunma
- Agency: Wenderoth, Lind & Ponack, L.L.P.
- Priority: JP2015-229896 20151125
- Main IPC: C12N15/85
- IPC: C12N15/85 ; C12N15/90 ; C07K16/18 ; C12N5/10 ; C12N9/10 ; C12N15/09 ; C07K16/44 ; C12N1/00 ; C12N15/11

Abstract:
A DNA methylation editing kit comprises: (1) a fusion protein of inactivated CRISPR-associated endonuclease Cas9 (dCas9) having no nuclease activity and a tag peptide array in which plural tag peptides are linked by linkers, or an RNA or DNA coding therefor; (2) a fusion protein(s) of a tag peptide-binding portion and a methylase or demethylase, or an RNA(s) or DNA(s) coding therefor; and (3) a guide RNA(s) (gRNA(s)) comprising a sequence complementary to a DNA sequence within 1 kb of a desired site of methylation or demethylation, or a DNA(s) expressing the gRNA(s).
Information query
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