Invention Grant
- Patent Title: Genetically-mutated bacterial strain for detecting estrogenic compound and method for detecting estrogenic compound using the same
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Application No.: US16146677Application Date: 2018-09-28
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Publication No.: US11591632B2Publication Date: 2023-02-28
- Inventor: Jin-Won Lee , Myung Chan Gye , Su-Hyun Ryu , Young-Pil Kim
- Applicant: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY
- Applicant Address: KR Seoul
- Assignee: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY
- Current Assignee: INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY
- Current Assignee Address: KR Seoul
- Agency: Sughrue Mion, PLLC
- Priority: KR10-2016-0038444 20160330
- Main IPC: C12N15/74
- IPC: C12N15/74 ; C12Q1/02 ; C07K14/47 ; C12N15/70 ; C07K14/72 ; C07K14/245 ; C07K14/195 ; G01N33/52

Abstract:
The present invention relates to a genetically mutated bacteria strain for detecting an estrogenic compound and a method for detecting an estrogenic compound by using the same. More specifically, the present invention relates to a bacteria strain having an ability to detect an estrogenic compound, transformed by plasmid A comprising base sequences in which a gene for encoding a coactivator interacting with an estrogen receptor ligand binding domain (ER LBD) is conjugated to a gene for encoding λCI protein, and plasmid B in which a gene for encoding an estrogen receptor ligand binding domain (ER LBD) is conjugated to a gene for encoding αNTD protein, and a method for detecting an estrogenic compound by using same.
The present invention can provide genetically mutated bacteria for detecting an estrogenic compound and a method for detecting an estrogenic compound by using same since the bacteria are based on estrogen receptor protein originated from the human body, and thus are environmentally friendly, and the detection of the bacteria can be performed in a very short time with low cost and labor by virtue of a relatively simple process.
The present invention can provide genetically mutated bacteria for detecting an estrogenic compound and a method for detecting an estrogenic compound by using same since the bacteria are based on estrogen receptor protein originated from the human body, and thus are environmentally friendly, and the detection of the bacteria can be performed in a very short time with low cost and labor by virtue of a relatively simple process.
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