In some examples, the disclosure describes a nano-position stage, an imaging device coupled to the nano-position stage, and a controller comprising instructions to: instruct the imaging device to capture a first image of a subject, instruct the nano-position stage to alter a position of the imaging device a distance based on a pixel size of the first image, instruct the imaging device to capture a second image of the subject at the altered position, and generate a third image of the subject utilizing the first image and the second image.