A cultivation method based on an optimization of a plant nitrogen fertilizer disclosure amount includes: selecting plants to be planted in this batch, recording a number of the plants planted this time, and preparing different parts of nitrogen fertilizer for individual plants, each part of the nitrogen fertilizer being 10 g; and planting the same batch of plants every Monday with an interval of one week and fertilizing the planted plants in the second week after planting. According to the cultivation method based on the optimization of the plant nitrogen fertilizer disclosure amount, by adopting a mode of more tests and less planting, consumed plant seedlings are correspondingly reduced when a worker calculates an optimal fertilizer disclosure amount, thus effectively avoiding excessive waste, making a cost of a subsequent use cheaper, bringing a help to a whole experiment, and enabling people to use the method more conveniently.