The present disclosure provides a method for treating a subject with periorbital puffiness by determining if the periorbital puffiness is due to edema or a structural change and administering a protein having hyaluronidase activity to the periorbital region of the subject if the periorbital puffiness is due to edema. Also provided are methods for determining if periorbital puffiness is due to edema or a structural change in a subject in need thereof by administering a composition that comprises a protein having hyaluronidase activity to the periorbital region of the subject, assessing the periorbital region at a predetermined amount of time after the administration of the composition, and then determining whether there is an improvement in the periorbital puffiness.