A personal protective equipment sanitizing assembly includes a cabinet that is comprised of a translucent material to pass light therethrough. An upper door is hingedly coupled to the cabinet and a lower door is hingedly coupled to the cabinet. A cart is rollably positioned in the cabinet and the cart is rollable onto the lower door when the lower door is opened for accessing the cart. A carousel unit is rotatably coupled to the cart and the carousel unit includes a plurality of suspensions to support a respective one of a plurality of face masks or other personal protective equipment. A plurality of light emitters is each coupled to the cart to emit light onto the carousel unit. Each of the light emitters has an operational wavelength ranging between approximately 10.0 nm and 400.0 nm for sterilizing the face masks.