Invention Grant
- Patent Title: Systems, methods and compositions for sequence manipulation with optimized functional CRISPR-Cas systems
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Application No.: US16592744Application Date: 2019-10-03
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Publication No.: US11597919B2Publication Date: 2023-03-07
- Inventor: Silvana Konermann , Alexandro Trevino , Mark Brigham , Fei Ran , Patrick Hsu , Chie-yu Lin , Osamu Nureki , Hiroshi Nishimasu , Ryuichiro Ishitani , Feng Zhang
- Applicant: The Broad Institute, Inc. , Massachusetts Institute of Technology , University of Tokyo , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
- Applicant Address: US MA Cambridge; US MA Cambridge; JP Tokyo; US MA Cambridge
- Assignee: The Broad Institute, Inc.,Massachusetts Institute of Technology,University of Tokyo,PRESIDENT AND FELLOWS OF HARVARD COLLEGE
- Current Assignee: The Broad Institute, Inc.,Massachusetts Institute of Technology,University of Tokyo,PRESIDENT AND FELLOWS OF HARVARD COLLEGE
- Current Assignee Address: US MA Cambridge; US MA Cambridge; JP Tokyo; US MA Cambridge
- Agency: Foley & Lardner LLP
- Agent Sunit Talapatra
- Main IPC: C12N9/22
- IPC: C12N9/22 ; A61K38/46 ; A61K48/00 ; C12N15/113 ; C12N15/90 ; C12Q1/6876 ; A61P43/00
Abstract:
The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.
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