Invention Grant
- Patent Title: Binary vectors and uses of same
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Application No.: US16622037Application Date: 2018-06-12
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Publication No.: US11597939B2Publication Date: 2023-03-07
- Inventor: Fabio Pasin , Juan Antonio García Álvarez , Leonor Cecilia Bedoya Rojas , María del Carmen Simón Mateo , Diego Vicente Orzáez Calatayud , Juan Miguel Bernabé Orts
- Applicant: Juan Antonio García Álvarez
- Applicant Address: ES Madrid
- Assignee: Juan Antonio García Álvarez
- Current Assignee: Juan Antonio García Álvarez
- Current Assignee Address: ES Madrid
- Agency: FisherBroyles, LLP
- Agent Andrew K. Gonsalves, Esq.
- Priority: ESES201730792 20170612
- International Application: PCT/ES2018/070421 WO 20180612
- International Announcement: WO2018/229319 WO 20181220
- Main IPC: C12N15/82
- IPC: C12N15/82

Abstract:
The invention relates to binary vectors based on compatible and autonomous origins, specifically based on the pBBR1 and RK2 replication origins. These binary vectors are useful for having a wide range of hosts, for their maintenance in Agrobacterium sp. and Escherichia coli, and as a new tool for plant synthetic biology as well as a flexible framework for assembly, transfer and characterization of multiple DNA elements. The binary vectors disclosed are small, preferably less than 3.8 kb in size, stable, include an origin compatible with the most commonly used binary T-DNA vectors, comply with current standards for plant synthetic biology, and allow the administration of multiple T-DNA cassettes by means of the multiplexing of the vectors. The present invention also relates to methods for transferring and expressing nucleic acid sequences using said binary vectors, and to the uses of the same.
Public/Granted literature
- US20200255845A1 BINARY VECTORS AND USES OF SAME Public/Granted day:2020-08-13
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