Invention Grant
- Patent Title: DNA assembly method and its application
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Application No.: US16724679Application Date: 2019-12-23
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Publication No.: US11597958B2Publication Date: 2023-03-07
- Inventor: Shuwen Liu , Tingyi Wen , Yun Zhang , Aihua Deng
- Applicant: Nanjing Zhongkeyouzi Institute of Biotechnology Co., Ltd.
- Applicant Address: CN Jiangsu
- Assignee: Nanjing Zhongkeyouzi Institute of Biotechnology Co., Ltd.
- Current Assignee: Nanjing Zhongkeyouzi Institute of Biotechnology Co., Ltd.
- Current Assignee Address: CN Jiangsu
- Agency: Panitch Schwarze Belisario & Nadel LLP
- Agent Stephany G. Small; Travis W. Bliss
- Priority: CN201811613376.4 20181227
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12N15/11
Abstract:
The invention relates to a plasmid, a DNA assembly method and its application recombinant strain. The plasmid has single adjacent Type IIP and Type IIS RE recognition sites. The plasmid combines the properties of Type IIP and Type IIS REs to achieve recursive cycling, SCAR-free and repeat sequence assembly.
Public/Granted literature
- US20200208188A1 DNA ASSEMBLY METHOD AND ITS APPLICATION Public/Granted day:2020-07-02
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