Disclosed herein are systems and methods for assessing an impact of malicious software causing a denial of service of components of industrial automation and control systems (IACS). In one aspect, an exemplary method comprises, generating a configuration of the IACS on a testing device based on specifications, obtaining a set of investigated software, where the set includes at least one sample of one malicious software, testing the generated configuration using the received set of investigated software, identifying occurrences of denials of service of the components of the testing device which are used to simulate the generated configuration, determining an impact of the malicious software on the generated configuration, and a degree of degradation of a performance of the generated configuration of IACS, and pronouncing a verdict as to a danger of the malicious software for the generated configuration of IACS based on the determined impact of the malicious software.