A self-service operating system and method, and an operation door. The system comprises: a control server, one or more operation doors, at least one robot, and one or more storage containers, wherein the control server is configured to respond to an item operation instruction, determine a target storage container and a target operation door which corresponds to an item to be operated, and send a carrying instruction to the robot; the robot is configured to respond to the carrying instruction to carry the target storage container to the target operation door, and a plurality of box openings on the target storage container respectively correspond to a plurality of box openings on the target operation door on a one-to-one basis; and the control server is further configured to control a box opening door of a target box opening on the target operation door to open.