An image sensing method, applicable to the anti-spoofing recognition of under screen optical fingerprint sensing, is provided, including: dividing the image sensor into sensing blocks, dividing the display area of the display device correspondingly according to the sensing area, and the display area including the light-emitting area; defining the luminous color of each display area and the color coordinate value of each luminous color; each sensing block sensing the light intensity of the image reflected to the sensing block from the display block emitting the light onto the reference object and the test object to be measured; calculating the anti-spoofing reference color information of the reference object and registering in the system; when sensing the fingerprint image, first obtaining the light intensity of each block, then calculating the color information of the test object; and finally, comparing the color information with the registered anti-spoofing reference color information.