Invention Grant
- Patent Title: Trace reconstruction from reads with indeterminant errors
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Application No.: US16105349Application Date: 2018-08-20
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Publication No.: US11600360B2Publication Date: 2023-03-07
- Inventor: Sergey Mikhailovich Yekhanin , Miklos Zoltan Racz
- Applicant: Microsoft Technology Licensing, LLC
- Applicant Address: US WA Redmond
- Assignee: Microsoft Technology Licensing, LLC
- Current Assignee: Microsoft Technology Licensing, LLC
- Current Assignee Address: US WA Redmond
- Agency: Newport IP, LLC
- Agent Benjamin A. Keim
- Main IPC: G16B30/00
- IPC: G16B30/00 ; G16B40/00 ; G16B40/20 ; G06F40/279 ; G06F40/166

Abstract:
Polynucleotide sequencing generates multiple reads of a polynucleotide molecule. Many or all of the reads contain errors. Trace reconstruction takes multiple reads generated by a polynucleotide sequencer and uses those multiple reads to reconstruct accurately the nucleotide sequence of the polynucleotide molecule. Some reads may contain errors that cannot be corrected. Thus, there may be reads that can be used throughout their entire length and other reads that have indeterminant errors which cannot be corrected. Rather than discarding the entire read when an indeterminant error is found, the portion of the read with the error is skipped and the sequence of the read following the error is used to reconstruct the trace. The amount of the read skipped is determined by the location of subsequence after the error that matches a consensus sequence of the other reads. Analysis resumes at a location determined by the location of the match.
Public/Granted literature
- US20200057838A1 TRACE RECONSTRUCTION FROM READS WITH INDETERMINANT ERRORS Public/Granted day:2020-02-20
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