A method and apparatus for transmitting and receiving a signal in a wireless communication system, according to an embodiment of the present invention, transmit a PRACH through a specific RO from among a plurality of ROs on the basis of a selected SSB, wherein a starting RB index of the specific RO may be determined on the basis of (i) the lowest RB index of an RB set including the specific RO, (ii) a starting RB index of an RO located at the lowest frequency, and (iii) the lowest RB index of an RB set including the RO located at the lowest frequency.