The present disclosure provides a light bar, a backlight assembly and a display device. The light bar includes a substrate; a first metal layer disposed on a first side of the substrate and comprising a plurality of first metal segments disposed at intervals along a length direction of the light bar; a second metal layer disposed on a second side opposite to the first side of the substrate and comprising a plurality of second metal segments disposed at intervals along the length direction of the light bar, a projection of the interval between two adjacent first metal segments on the substrate falls into projections of the second metal segments on the substrate, and a projection of the interval between two adjacent second metal segments on the substrate falls into projections of the first metal segments on the substrate.