Proposed are a display substrate, a control method thereof and a wearable display device. The display substrate includes M rows and N columns of pixels and 2M rows of scanning lines, a first sub-pixel and a second sub-pixel of pixels in an i-th row are connected to the same scanning line and electrically connected to one of a (2i−1)-th row of scanning line and a 2i-th row of scanning line, and a third sub-pixel of the pixels in the i-th row is electrically connected to the other of the (2i−1)-th row of scanning line and the 2i-th row of scanning line; when the display substrate is displaying, a first sub-pixel, a second sub-pixel and a third sub-pixel among two pixels located in a (2i−1)-th row, a j-th column, and a 2i-th row, a j-th column emit light at an i-th stage of each frame.