A display panel and a method for manufacturing the display panel are provided. The display panel includes a plurality of pixel units, wherein each of the pixel units includes first sub-pixels, second sub-pixels, third sub-pixels, first pixel definition layers, and a second pixel definition layer. Each of the pixel units is enclosed by one of the first pixel definition layers into a rectangle; and the second pixel definition layer is disposed in each of the first pixel definition layers and between the first sub-pixels, the second sub-pixels, and the third sub-pixels.