The present disclosure relates to the isolation, identification and characterization of novel sporeforming probiotic strain(s) with NSPase (Non-Starch Polysaccharides-active hydrolases) activity isolated from fish gut microbiota, methods and uses thereof. The sporeforming probiotic strain(s) with NSPase activity now disclosed are able of producing carbohydrate-active enzymes (CAZymes) that hydrolyse non-starch polysaccharides (NSPs) and accesses their potential as probiotics (PRO) for use in particular in aquafeeds.