The subject matter described herein includes methods, systems, and computer readable media for stateless service traffic generation. A method for stateless service traffic generation occurs at a network equipment test system. The method includes generating, at a first transmit port associated with the network equipment test system, a first test packet flow comprising one or more packets, wherein the first test packet flow indicates a match and action instruction for triggering an action at a second transmit port associated with the network equipment test system; sending the first test packet flow toward a node associated with a data center under test (DCUT); receiving the first test packet flow from the node associated with the DCUT; and performing, using the match and action instruction, the action at the second transmit port associated with the network equipment test system.