Check valve assemblies for fluid flow sets and devices are provided. The check valve assembly includes an inlet body including a fluid inlet, first and second seal beads, and a central channel. An outlet body includes a fluid outlet and a stem having a centering post, the centering post received by the central channel of the inlet body. A seal includes inner and outer rings, wherein the inner ring is configured to engage with the first seal bead and the outer ring is configured to engage with the second seal bead in a fully sealed position of the check valve assembly. Fluid flow sets and methods for manufacturing check valve assemblies are also provided.