What is disclosed in the present invention is to provide a quantitative transfer pipette structure, wherein an anti-backflow compartment is configured in the first bulb. The dimensions and locations of the first bulb, the second bulb and the third bulb are specifically configured to aspirate and transfer a specific volume of the liquid to a container, mix the liquid with the substance in the container by pressing and releasing the specific bulbs, so as to achieve the effects of precise quantification and sufficient mixing.