One example provides a system for reading birefringent data. The system comprises one or more light sources, a first polarization state generator positioned to generate first polarized light from light of a first wavelength band output by the one or more light sources, a second polarization state generator positioned to generate second polarized light from light of a second wavelength band output by the one or light sources, an image sensor configured to acquire an image of the sample region via the first polarized light and the second polarized light, a polarization state analyzer disposed between the sample region and the image sensor, a first bandpass filter configured to pass light of the first wavelength band onto the image sensor, and a second bandpass filter configured to pass light of the second wavelength band onto the image sensor.