This disclosure relates generally to a system and method for real-time non-invasive estimation of food quality within enclosed package. Existing works utilize invasive methods that require direct contact of the food item with the sensors. In the present disclosure, a potential is applied over a plurality of frequencies through the food item contained the enclosed package which includes a plurality of polyethylene layers and a conducting layer arranged between two adjacent polyethylene layers using electrochemical impedance spectroscopy. Values of electrical voltages and the electrical impedances of the food item are then obtained. A plurality of features is derived from the obtained values of the electrical voltages and the electrical impedances using a trained model. The present disclosure estimates the quality of the food item in real-time by co-relating the plurality of derived features with the quality of the food item contained inside the enclosed package.