Invention Grant
- Patent Title: Methods of producing nucleic acid libraries and compositions and kits for practicing same
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Application No.: US17058066Application Date: 2019-06-05
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Publication No.: US11629345B2Publication Date: 2023-04-18
- Inventor: Richard Green , Joshua Kapp
- Applicant: The Regents of the University of California
- Applicant Address: US CA Oakland
- Assignee: The Regents of the University of California
- Current Assignee: The Regents of the University of California
- Current Assignee Address: US CA Oakland
- Agency: Bozicevic, Field & Francis LLP
- Agent Brian E. Davy
- International Application: PCT/US2019/035617 WO 20190605
- International Announcement: WO2019/236726 WO 20191212
- Main IPC: C12N15/00
- IPC: C12N15/00 ; C12N15/10 ; C12Q1/6869

Abstract:
Provided are methods of producing nucleic acid libraries. The methods include combining single-stranded nucleic acid binding protein-bound single-stranded nucleic acid (SSB-bound ssNA), an adapter oligonucleotide, and a splint oligonucleotide, to form complexes including the splint oligonucleotide hybridized to a terminal region of the SSB-bound ssNA and to the adapter oligonucleotide. An end of the first adapter oligonucleotide is adjacent to an end of the first terminal region of the SSB-bound ssNA, and the methods may further include covalently linking the adjacent ends. Also provided are compositions and kits that find use, e.g., in practicing the methods of the present disclosure.
Public/Granted literature
- US20210222161A1 Methods of Producing Nucleic Acid Libraries and Compositions and Kits for Practicing Same Public/Granted day:2021-07-22
Information query
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