A bioptic scanner optical arrangement with a single sensor split four ways is disclosed herein. An example bioptic scanner optical arrangement includes a housing, an imaging assembly having a primary FOV, a decode module, a generally horizontal window supported by the housing, a generally upright window supported by the housing, and a mirror arrangement positioned within the interior region. The mirror arrangement is configured to divide the primary FOV into a plurality of subfields, to redirect at least two of the plurality of subfields through the generally horizontal window, and to redirect at least another two of the plurality of subfields through the generally upright window. The bioptic barcode reader has no other imaging assembly communicatively coupled to the decode module and used to process images for decoding indicia.