A spot sub-pixel center positioning method based on pixel movement and cutting, the method includes driving the CCD image plane to move along the x direction and the y direction through a displacement platform, selecting target pixels to move and cut the spot, and recording the gray value change of the target pixels, constructing the mapping relationship between gray value and displacement, performing differential transformation on the gray value and displacement curve, and finally performing interpolation fitting to obtain the sub-pixel coordinate value of the center point of the light spot. The measurement accuracy can be maintained within ½ of the single-step displacement of cutting. The method is not only suitable for strong and weak distribution of light spots, but also for fully saturated light spots, and also for asymmetrically distributed and blurred edges. It also has a good measurement effect and can handle a variety of spot scenes.