A display substrate, a display device and a test method of the display substrate are disclosed. The display substrate includes a display region and a peripheral region. The peripheral region includes: a first leading wire extending in a first direction and including a first end and a second end; a first test wire electrically connected with the first leading wire at a first position of the first test wire between the first end and the second end; the display region includes first signal wires of first group extending in a second direction, two first signal wires arranged outermost in the first direction among the first signal wires of first group are respectively connected with the first end and the second end, and remaining first signal wires among the first signal wires of first group are connected with the first leading wire between the first end and the second end.