A cannula holder capable of securing a cannula to the holder at the time of piercing work, and easily removing the cannula from the holder using tweezers or the like. The cannula holder, to which a piercing needle for piercing an eyeball and a cannula are attached when attaching the cannula used in ophthalmic operations to the eyeball, includes claw portions that are formed on a front end of the cannula holder and which press sides of the cannula either at positions where said claw portions face each other or from multiple directions. A depression may be provided in a side of the front end of the cannula holder, allowing visibility of a part of a back end of the cannula from the depression.