Invention Grant
- Patent Title: Composition comprising lactic acid bacteria improved in intestinal adherence by coating with silk fibroin
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Application No.: US16500952Application Date: 2018-04-05
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Publication No.: US11642318B2Publication Date: 2023-05-09
- Inventor: Bohye Heo , Youil Kim , Woolee Kim , Minho Seo , Byoungkook Kim , Insuk Choi
- Applicant: CKD Bio Corp.
- Applicant Address: KR Seoul
- Assignee: CKD Bio Corp.
- Current Assignee: CKD Bio Corp.
- Current Assignee Address: KR Seoul
- Agency: Harness, Dickey & Pierce, P.L.C.
- Priority: KR 20170045326 2017.04.07
- International Application: PCT/KR2018/004026 2018.04.05
- International Announcement: WO2018/186700A 2018.10.11
- Date entered country: 2019-10-04
- Main IPC: A61K9/00
- IPC: A61K9/00 ; A61K9/50 ; A61P1/00 ; A61K35/744 ; A61K35/745 ; A61K35/747 ; C12N1/20 ; C12N11/02 ; C12N11/12 ; A61K35/00

Abstract:
The present invention relates to a method for improving lactic acid bacteria in survival rate, storage stability, resistance to acid or bile, and adherence to intestinal epithelial cells by coating the surface of lactic acid bacteria with silk fibroin, and a lactic acid bacteria composition prepared therethrough. Conventional techniques construct only a physical protective barrier outside a lactic acid bacteria body by multi-stage coating and thus retain the limitation of being unable to identify an effect on the coherence of lactic acid bacteria to intestinal epithelial cells upon the uptake of the lactic acid bacteria. In contrast, the present invention provides a method in which lactic acid bacteria is coated with silk fibroin extracted from cocoons, whereby the lactic acid bacteria is improved in stability under a storage and distribution condition as well as having remarkably increased stability and intestinal adherence particularly under an intestinal environment.
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