The present disclosure provides a phase shifter and a manufacturing method thereof, and an antenna. The phase shifter includes a first substrate including a reference electrode, and a second substrate including a delay line and a bias line. An orthographic projection of the bias line on the first substrate and that of the delay line, at least partially overlap with that of the reference electrode respectively. The orthographic projection of the bias line on the first substrate does not overlap with that of the delay line. The delay line is used for transmitting a microwave signal and defining a microwave transmission region. When electrical signals are applied to the reference electrode, the delay line and the bias line, electric field areas formed respectively between the reference electrode and the delay line, and between the reference electrode and the bias line, cover the microwave transmission region.