A method of calibrating an image sensor includes obtaining a plurality of multi-light source images generated from a plurality of image sensor modules, wherein each of the plurality of image sensor modules generates at least three multi-light source images; obtaining, based on the plurality of multi-light source images, a plurality of crosstalk levels and a plurality of color-specific correction coefficients; generating modeling data based on a relationship between a crosstalk level for a first color and the plurality of color-specific correction coefficients; and obtaining, based on a single light source image captured by a first image sensor module and the modeling data, a color-specific correction coefficient for a target pixel of an image sensor, the image sensor being provided in the first image sensor module, and the color-specific correction coefficient being usable for correcting a crosstalk of the target pixel.