A method for closing an opening in biological tissue of intra-abdominal structures, which includes the steps of (i) providing a tissue closure system that includes a suture guide sub-system for engaging tissue disposed proximate the tissue opening and a suture passer sub-system that slideably engages the suture guide sub-system, (ii) inserting the suture guide sub-system into and through a tissue opening, (iii) inserting the suture passer sub-system with a section of suture engaged thereto into and through a first tissue region, (iv) rotating the suture guide sub-system, (v) inserting the suture passer sub-system with another section of suture engaged thereto into and through a second tissue region, (vi) withdrawing suture guide sub-system, (vii) releasing both sections of the suture captured by the suture guide sub-system, and (viii) drawing both sections of the suture together, wherein the first and second tissue regions are drawn together and the tissue opening is closed.